Genetic Characterization of Drought-Tolerant Maize Genotypes Based on SSR Markers
نویسندگان
چکیده
Using 20 pairs of SSR primers situated on each chromosome the maize genetic makeup, 27 drought-tolerant genotypes obtained from Germplasm Unit International Institute Tropical Agriculture (IITA), Ibadan, Nigeria, were examined for diversity. The samples' genomic DNA (gDNA) was extracted using CTAB procedure specified by CIMMYT (2005). All markers used in this investigation extremely polymorphic, resulting 122 repeatable and polymorphic bands that scored with an average 12.20 alleles. Each primer yielded a main allele frequency no more than 0.5. major 10 0.3036. With 0.8334, all had gene diversity values greater 0.6. Similar to this, Polymorphic Information Content (PIC) 0.8164 varied 0.9051 (primer umc1042) 0.6639 (Umc1669). Since umc1042 best identified variability, alleles generated points presence significant molecular variation across samples under study. This occurrence is further supported (GD) polymorphism information content primers. Therefore, it can be inferred are appropriate genotyping Due their different relationships, varieties IWD C2 SYN F2 SAMMAZ 52 have most diversified utilised as parent materials breeding programme.
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ژورنال
عنوان ژورنال: Nigerian Journal of Biotechnology
سال: 2023
ISSN: ['0189-1731']
DOI: https://doi.org/10.4314/njb.v39i2.12